1172 Targeting skin photoaging with okra seed extracts

Ultraviolet (UV) radiation is known to cause oxidative stress and DNA damage the skin, leads premature aging. Recently, anti-aging molecules have been attracting unprecedented attention where natural ingredients present in nutraceuticals become latest trend. In this study, Abelmoschus Esculentus (i.e., okra, lady fingers) seeds (OS) were extracted using a pressurized hot water “green” method. OS extracts shown contain polyphenolic compounds exert potent anti-oxidant activityin vitro. We further examined their potential effects both 2D culture of human primary keratinocytes (HPKs) 3D organotypic skin models exposed UV. The analyzed at mRNA protein levels for differentiation senescence biomarkers, inflammation biomarkers. Clonogenicity assays also conducted evaluate HPK stemness after treatment. Our data showed that 0.01 mg/mL exerted an effect preventing UV-induced late inflammation. addition, clonogenicity limited loss proliferative capacities HPKs UV exposure, suggesting enhanced maintenance stemness. Consistently, our 0.05 prevented upper epidermal layers occurring exposure. Lastly, induced by was as evidenced Lamin B1 suprabasal layers, while exposure its loss. conclusion, demonstrated significant photoaging homeostasis skin.